AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 246: G80-G85, 1984;
0193-1857/84 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Thornburg, W.
Right arrow Articles by Koldovsky, O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thornburg, W.
Right arrow Articles by Koldovsky, O.

AJP - Gastrointestinal and Liver Physiology, Vol 246, Issue 1 80-G85, Copyright © 1984 by American Physiological Society

ARTICLES

Gastrointestinal absorption of epidermal growth factor in suckling rats

W. Thornburg, L. Matrisian, B. Magun and O. Koldovsky

Epidermal growth factor (EGF) was detected in the milk of adult lactating Sprague-Dawley female rats (38.85 ng/ml) and in the stomach (37.25 ng/g content) and plasma (32.36 ng/ml) of 13-day-old suckling offspring. Sixty-nanogram (0.12 mCi/ml) doses of 125I-EGF were administered orally to sucklings in 200 microliters of buffer for 0, 0.5, 1.0, and 3.0 h. Lung, liver kidney, brain, and blood each contained 1% or less of the administered radioactivity. Stomach wall (8%) and content (63%), intestinal wall (15%) and flushing (38%), and skin (18%) contained larger amounts reaching maxima in these three regions at 0, 0.5, and 3.0 h, respectively. Except for skin, a substantial amount of radioactivity from all tissues represented intact (6-90%) and immunoreactive (3-90%) EGF based on Sephadex G-25 chromatography and anti-EGF antibody binding, respectively. From 30 to 55% of the radioactivity from wall (gastric or small intestinal) or lumina was also capable of binding to A-431 cell surface receptors. Isoelectric points of major species found in stomach (4.2), intestine (4.1), and other tissues differed from that of administered EGF (4.5).


This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
T. Kinouchi, K. Koizumi, T. Kuwata, and T. Yajima
Crucial role of milk-borne insulin in the development of pancreatic amylase at the onset of weaning in rats
Am J Physiol Regulatory Integrative Comp Physiol, December 1, 1998; 275(6): R1958 - R1967.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
B. Dvorak, M. D. Halpern, H. Holubec, C. S. Williams, D. L. McWilliam, J. A. Dominguez, R. Stepankova, C. M. Payne, and R. S. McCuskey
Epidermal growth factor reduces the development of necrotizing enterocolitis in a neonatal rat model
Am J Physiol Gastrointest Liver Physiol, January 1, 2002; 282(1): G156 - G164.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online