AJP - Gastrointestinal and Liver Physiology, Vol 246, Issue 2 187-G194, Copyright © 1984 by American Physiological Society
Effect of chloroquine on low-density lipoprotein catabolic pathway in rat hepatocytes
C. A. Hornick, A. L. Jones, G. Renaud, G. Hradek and R. J. Havel
Injection of 125I-low-density lipoprotein (LDL) into estradiol-treated rats
substantially increases the volume of the multivesicular body compartment
in hepatocytes. When these animals were additionally given chloroquine 60
and 120 min before injection of 125I-LDL, the amount of 125I retained by
the liver increased threefold and the amount of label in multivesicular
bodies increased fivefold, as determined by quantitative analysis of
autoradiograms. Lamellar inclusions that appeared after chloroquine also
contained a substantial fraction of 125I, whereas the fraction of 125I in
secondary lysosomes was reduced. From these data it is concluded that the
multivesicular bodies comprise an intermediate endocytic compartment, the
conversion of which to secondary lysosomes is impeded by chloroquine.
