AJP - GI Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Gastrointest Liver Physiol 246: G204-G208, 1984;
0193-1857/84 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dharmsathaphorn, K.
Right arrow Articles by Masui, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dharmsathaphorn, K.
Right arrow Articles by Masui, H.

AJP - Gastrointestinal and Liver Physiology, Vol 246, Issue 2 204-G208, Copyright © 1984 by American Physiological Society

ARTICLES

A human colonic tumor cell line that maintains vectorial electrolyte transport

K. Dharmsathaphorn, J. A. McRoberts, K. G. Mandel, L. D. Tisdale and H. Masui

A human colonic epithelial cell line, T84, derived from a colonic carcinoma, has been examined both morphologically and functionally. The cells grew to confluence as a monolayer with the basolateral membrane attached to the surface of the culture dish and a microvillus-studded apical membrane facing the media. Tight junctions and desmosomes were demonstrated between adjacent cells. Confluent monolayer cultures conducted vectorial electrolyte transport that could be altered by a variety of secretagogues and antisecretagogues similar to isolated intestinal tissues. This cell line will serve as an excellent model system for the study of electrolyte transport processes and their regulation by peptide hormones and neurotransmitters.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
R. T. Worrell, L. Merk, and J. B. Matthews
Ammonium transport in the colonic crypt cell line, T84: role for Rhesus glycoproteins and NKCC1
Am J Physiol Gastrointest Liver Physiol, February 1, 2008; 294(2): G429 - G440.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. Q. van der Merwe, M. D. Hollenberg, and W. K. MacNaughton
EGF receptor transactivation and MAP kinase mediate proteinase-activated receptor-2-induced chloride secretion in intestinal epithelial cells
Am J Physiol Gastrointest Liver Physiol, February 1, 2008; 294(2): G441 - G451.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
P. J. Bajwa, A. Alioua, J. W. Lee, D. S. Straus, L. Toro, and C. Lytle
Fenofibrate inhibits intestinal Cl secretion by blocking basolateral KCNQ1 K+ channels
Am J Physiol Gastrointest Liver Physiol, December 1, 2007; 293(6): G1288 - G1299.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. Y. C. Chow and K. E. Barrett
Role of protein phosphatase 2A in calcium-dependent chloride secretion by human colonic epithelial cells
Am J Physiol Cell Physiol, January 1, 2007; 292(1): C452 - C459.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
R. T. Worrell, A. Best, O. R. Crawford, J. Xu, M. Soleimani, and J. B. Matthews
Apical ammonium inhibition of cAMP-stimulated secretion in T84 cells is bicarbonate dependent
Am J Physiol Gastrointest Liver Physiol, October 1, 2005; 289(4): G768 - G778.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. M. Smith, P. A. Johanesen, M. K. Wendt, D. G. Binion, and M. B. Dwinell
CXCL12 activation of CXCR4 regulates mucosal host defense through stimulation of epithelial cell migration and promotion of intestinal barrier integrity
Am J Physiol Gastrointest Liver Physiol, February 1, 2005; 288(2): G316 - G326.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. B. Dwinell, H. Ogawa, K. E. Barrett, and M. F. Kagnoff
SDF-1/CXCL12 regulates cAMP production and ion transport in intestinal epithelial cells via CXCR4
Am J Physiol Gastrointest Liver Physiol, May 1, 2004; 286(5): G844 - G850.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
R. F. Dubin, S. K. Robinson, and J. H. Widdicombe
Secretion of lactoferrin and lysozyme by cultures of human airway epithelium
Am J Physiol Lung Cell Mol Physiol, April 1, 2004; 286(4): L750 - L755.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
R. T. Worrell, J. Oghene, and J. B. Matthews
Ammonium effects on colonic Cl- secretion: anomalous mole fraction behavior
Am J Physiol Gastrointest Liver Physiol, January 1, 2004; 286(1): G14 - G22.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
G. Melmed, L. S. Thomas, N. Lee, S. Y. Tesfay, K. Lukasek, K. S. Michelsen, Y. Zhou, B. Hu, M. Arditi, and M. T. Abreu
Human Intestinal Epithelial Cells Are Broadly Unresponsive to Toll-Like Receptor 2-Dependent Bacterial Ligands: Implications for Host-Microbial Interactions in the Gut
J. Immunol., February 1, 2003; 170(3): 1406 - 1415.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
K. Howe, J. Gauldie, and D. M. McKay
TGF-beta effects on epithelial ion transport and barrier: reduced Cl- secretion blocked by a p38 MAPK inhibitor
Am J Physiol Cell Physiol, December 1, 2002; 283(6): C1667 - C1674.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. T. Abreu, E. T. Arnold, L. S. Thomas, R. Gonsky, Y. Zhou, B. Hu, and M. Arditi
TLR4 and MD-2 Expression Is Regulated by Immune-mediated Signals in Human Intestinal Epithelial Cells
J. Biol. Chem., May 31, 2002; 277(23): 20431 - 20437.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. T. Abreu, E. T. Arnold, J. Y. C. Chow, and K. E. Barrett
Phosphatidylinositol 3-Kinase-dependent Pathways Oppose Fas-induced Apoptosis and Limit Chloride Secretion in Human Intestinal Epithelial Cells. IMPLICATIONS FOR INFLAMMATORY DIARRHEAL STATES
J. Biol. Chem., December 7, 2001; 276(50): 47563 - 47574.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M. Ciarlet, S. E. Crawford, and M. K. Estes
Differential Infection of Polarized Epithelial Cell Lines by Sialic Acid-Dependent and Sialic Acid-Independent Rotavirus Strains
J. Virol., December 1, 2001; 75(23): 11834 - 11850.
[Abstract] [Full Text]

Home page
 J Med MicrobiolHome page
A. TAKAHASHI, T. IIDA, R. NAIM, Y. NAYKAYA, and T. HONDA
Chloride secretion induced by thermostable direct haemolysin of Vibrio parahaemolyticus depends on colonic cell maturation
J. Med. Microbiol., October 1, 2001; 50(10): 870 - 878.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
R. Toriano, A. Kierbel, M. A. Ramirez, G. Malnic, and M. Parisi
Spontaneous water secretion in T84 cells: effects of STa enterotoxin, bumetanide, VIP, forskolin, and A-23187
Am J Physiol Gastrointest Liver Physiol, September 1, 2001; 281(3): G816 - G822.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
C. J. Watson, M. Rowland, and G. Warhurst
Functional modeling of tight junctions in intestinal cell monolayers using polyethylene glycol oligomers
Am J Physiol Cell Physiol, August 1, 2001; 281(2): C388 - C397.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. T. Abreu, P. Vora, E. Faure, L. S. Thomas, E. T. Arnold, and M. Arditi
Decreased Expression of Toll-Like Receptor-4 and MD-2 Correlates with Intestinal Epithelial Cell Protection Against Dysregulated Proinflammatory Gene Expression in Response to Bacterial Lipopolysaccharide
J. Immunol., August 1, 2001; 167(3): 1609 - 1616.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
N. Chang, J. M. Uribe, S. J. Keely, S. Calandrella, and K. E. Barrett
Insulin and IGF-I inhibit calcium-dependent chloride secretion by T84 human colonic epithelial cells
Am J Physiol Gastrointest Liver Physiol, July 1, 2001; 281(1): G129 - G137.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
A. C. Zambon, L. L. Brunton, K. E. Barrett, R. J. Hughes, B. Torres, and P. A. Insel
Cloning, Expression, Signaling Mechanisms, and Membrane Targeting of P2Y11 Receptors in Madin Darby Canine Kidney Cells
Mol. Pharmacol., July 1, 2001; 60(1): 26 - 35.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. E. Smitham and K. E. Barrett
Differential effects of apical and basolateral uridine triphosphate on intestinal epithelial chloride secretion
Am J Physiol Cell Physiol, June 1, 2001; 280(6): C1431 - C1439.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
C. S. Bockman, M. E. Bradley, H. K. Dang, W. Zeng, M. A. Scofield, and F. J. Dowd
Molecular and Pharmacological Characterization of Muscarinic Receptor Subtypes in a Rat Parotid Gland Cell Line: Comparison with Native Parotid Gland
J. Pharmacol. Exp. Ther., April 12, 2001; 297(2): 718 - 726.
[Abstract] [Full Text]

Home page
 J. Am. Soc. Nephrol.Home page
A. ODERMATT, A. AUDIGÉ, C. FRICK, B. VOGT, B. M. FREY, F. J. FREY, and L. MAZZUCCHELLI
Identification of Receptor Ligands by Screening Phage-Display Peptide Libraries Ex Vivo on Microdissected Kidney Tubules
J. Am. Soc. Nephrol., February 1, 2001; 12(2): 308 - 316.
[Abstract] [Full Text]

Home page
 Clin. Cancer Res.Home page
M. E. S. Kahn, A. Senderowicz, E. A. Sausville, and K. E. Barrett
Possible Mechanisms of Diarrheal Side Effects Associated with the Use of a Novel Chemotherapeutic Agent, Flavopiridol
Clin. Cancer Res., February 1, 2001; 7(2): 343 - 349.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Cell Physiol.Home page
Z. Bebok, A. Tousson, L. M. Schwiebert, and C. J. Venglarik
Improved oxygenation promotes CFTR maturation and trafficking in MDCK monolayers
Am J Physiol Cell Physiol, January 1, 2001; 280(1): C135 - C145.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
P. J. Gunter-Smith, O. Abdulkadir, L. Hammonds-Odie, M. Scanlon, and R. Terrell
A primary culture of guinea pig gallbladder epithelial cells that is responsive to secretagogues
Am J Physiol Gastrointest Liver Physiol, November 1, 2000; 279(5): G866 - G874.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
S. Sartingen, E. Rozdzinski, A. Muscholl-Silberhorn, and R. Marre
Aggregation Substance Increases Adherence and Internalization, but Not Translocation, of Enterococcus faecalis through Different Intestinal Epithelial Cells In Vitro
Infect. Immun., October 1, 2000; 68(10): 6044 - 6047.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
A. K. Singh, B. D. Schultz, J. A. Katzenellenbogen, E. M. Price, R. J. Bridges, and N. A. Bradbury
Estrogen Inhibition of Cystic Fibrosis Transmembrane Conductance Regulator-Mediated Chloride Secretion
J. Pharmacol. Exp. Ther., October 1, 2000; 295(1): 195 - 204.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. V. Sitaraman, M. Si-Tahar, D. Merlin, G. R. Strohmeier, and J. L. Madara
Polarity of A2b adenosine receptor expression determines characteristics of receptor desensitization
Am J Physiol Cell Physiol, June 1, 2000; 278(6): C1230 - C1236.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
D. R. Halm and S. T. Halm
Secretagogue response of goblet cells and columnar cells in human colonic crypts1
Am J Physiol Cell Physiol, January 1, 2000; 278(1): C212 - C233.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. P. Colgan, R. M. Hershberg, G. T. Furuta, and R. S. Blumberg
Ligation of intestinal epithelial CD1d induces bioactive IL-10: Critical role of the cytoplasmic tail in autocrine signaling
PNAS, November 23, 1999; 96(24): 13938 - 13943.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
A. Quaroni, J. Q. Tian, M. Goke, and D. K. Podolsky
Glucocorticoids have pleiotropic effects on small intestinal crypt cells
Am J Physiol Gastrointest Liver Physiol, November 1, 1999; 277(5): G1027 - G1040.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
D. R. Halm and S. T. Halm
Secretagogue response of goblet cells and columnar cells in human colonic crypts
Am J Physiol Cell Physiol, September 1, 1999; 277(3): C501 - C522.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
S M Fish, R Proujansky, and W W Reenstra
Synergistic effects of interferon gamma and tumour necrosis factor alpha on T84 cell function
Gut, August 1, 1999; 45(2): 191 - 198.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C.-C. Shieh, B. K. Sadasivan, G. J. Russell, M. P. Schon, C. M. Parker, and M. B. Brenner
Lymphocyte Adhesion to Epithelia and Endothelia Mediated by the Lymphocyte Endothelial-Epithelial Cell Adhesion Molecule Glycoprotein
J. Immunol., August 1, 1999; 163(3): 1592 - 1601.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. T. Taylor, N. Fueki, A. Agah, R. M. Hershberg, and S. P. Colgan
Critical Role of cAMP Response Element Binding Protein Expression in Hypoxia-elicited Induction of Epithelial Tumor Necrosis Factor-alpha
J. Biol. Chem., July 2, 1999; 274(27): 19447 - 19454.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
K. E. Beauregard, S. Wimer-Mackin, R. J. Collier, and W. I. Lencer
Anthrax Toxin Entry into Polarized Epithelial Cells
Infect. Immun., June 1, 1999; 67(6): 3026 - 3030.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
A. Youakim and M. Ahdieh
Interferon-gamma decreases barrier function in T84 cells by reducing ZO-1 levels and disrupting apical actin
Am J Physiol Gastrointest Liver Physiol, May 1, 1999; 276(5): G1279 - G1288.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
C. G. Arvidson, R. Kirkpatrick, M. T. Witkamp, J. A. Larson, C. A. Schipper, L. S. Waldbeser, P. O'Gaora, M. Cooper, and M. So
Neisseria gonorrhoeae Mutants Altered in Toxicity to Human Fallopian Tubes and Molecular Characterization of the Genetic Locus Involved
Infect. Immun., February 1, 1999; 67(2): 643 - 652.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
R. V. Benya, J. A. Marrero, D. A. Ostrovskiy, A. Koutsouris, and G. Hecht
Human colonic epithelial cells express galanin-1 receptors, which when activated cause Cl- secretion
Am J Physiol Gastrointest Liver Physiol, January 1, 1999; 276(1): G64 - G72.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. Zareie, D. M. McKay, G. G. Kovarik, and M. H. Perdue
Monocyte/macrophages evoke epithelial dysfunction: indirect role of tumor necrosis factor-alpha
Am J Physiol Cell Physiol, October 1, 1998; 275(4): C932 - C939.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. K. Shaw, A. Hermanowski-Vosatka, T. Shibahara, B. A. McCormick, C. A. Parkos, S. L. Carlson, E. C. Ebert, M. B. Brenner, and J. L. Madara
Migration of intestinal intraepithelial lymphocytes into a polarized epithelial monolayer
Am J Physiol Gastrointest Liver Physiol, September 1, 1998; 275(3): G584 - G591.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. T. Turner, R. S. Redman, J. M. Camden, L. A. Landon, and D. O. Quissell
A rat parotid gland cell line, Par-C10, exhibits neurotransmitter-regulated transepithelial anion secretion
Am J Physiol Cell Physiol, August 1, 1998; 275(2): C367 - C374.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. Goke, M. Kanai, and D. K. Podolsky
Intestinal fibroblasts regulate intestinal epithelial cell proliferation via hepatocyte growth factor
Am J Physiol Gastrointest Liver Physiol, May 1, 1998; 274(5): G809 - G818.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
D. J. Philpott, D. M. McKay, W. Mak, M. H. Perdue, and P. M. Sherman
Signal Transduction Pathways Involved in Enterohemorrhagic Escherichia coli-Induced Alterations in T84 Epithelial Permeability
Infect. Immun., April 1, 1998; 66(4): 1680 - 1687.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
K. E. Barrett, J. Smitham, A. Traynor-Kaplan, and J. M. Uribe
Inhibition of Ca2+-dependent Cl- secretion in T84 cells: membrane target(s) of inhibition is agonist specific
Am J Physiol Cell Physiol, April 1, 1998; 274(4): C958 - C965.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
D. J. Philpott, C. A. Ackerley, A. J. Kiliaan, M. A. Karmali, M. H. Perdue, and P. M. Sherman
Translocation of verotoxin-1 across T84 monolayers: mechanism of bacterial toxin penetration of epithelium
Am J Physiol Gastrointest Liver Physiol, December 1, 1997; 273(6): G1349 - G1358.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. E. Huflejt, E. T. Jordan, M. A. Gitt, S. H. Barondes, and H. Leffler
Strikingly Different Localization of Galectin-3 and Galectin-4 in Human Colon Adenocarcinoma T84 Cells. GALECTIN-4 IS LOCALIZED AT SITES OF CELL ADHESION
J. Biol. Chem., May 30, 1997; 272(22): 14294 - 14303.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. M. Uribe, S. J. Keely, A. E. Traynor-Kaplan, and K. E. Barrett
Phosphatidylinositol 3-Kinase Mediates the Inhibitory Effect of Epidermal Growth Factor on Calcium-dependent Chloride Secretion
J. Biol. Chem., October 25, 1996; 271(43): 26588 - 26595.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Y. C. Chow, J. M. Uribe, and K. E. Barrett
A Role for Protein Kinase Cepsilon in the Inhibitory Effect of Epidermal Growth Factor on Calcium-stimulated Chloride Secretion in Human Colonic Epithelial Cells
J. Biol. Chem., July 7, 2000; 275(28): 21169 - 21176.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. T. Taylor, G. T. Furuta, K. Synnestvedt, and S. P. Colgan
Phosphorylation-dependent targeting of cAMP response element binding protein to the ubiquitin/proteasome pathway in hypoxia
PNAS, October 24, 2000; 97(22): 12091 - 12096.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. Lipecka, M. Bali, A. Thomas, P. Fanen, A. Edelman, and J. Fritsch
Distribution of ClC-2 chloride channel in rat and human epithelial tissues
Am J Physiol Cell Physiol, April 1, 2002; 282(4): C805 - C816.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online