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Am J Physiol Gastrointest Liver Physiol 247: G651-G661, 1984;
0193-1857/84 $5.00
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AJP - Gastrointestinal and Liver Physiology, Vol 247, Issue 6 651-G661, Copyright © 1984 by American Physiological Society

ARTICLES

A map of membrane populations isolated from rat exorbital gland

A. K. Mircheff and C. C. Lu

We combined separation procedures based on three independent physical properties, sedimentation coefficient, density, and partitioning in an aqueous polymer two-phase system, to generate a three-dimensional subcellular fractionation of rat exorbital lacrimal gland. The distributions of protein and five enzymatic markers define a total of 13 physically and biochemically distinct membrane populations. These include epithelial cell apical membranes, purified 330-fold with respect to the initial homogenate; basal-lateral membranes, purified 80-fold; mitochondria, purified 19-fold; and a major endoplasmic reticulum population, purified 22-fold. Also apparent is a major Golgi population, which is extensively overlapped by other membrane populations; two populations that can be visualized as forming transitions between the endoplasmic reticulum and Golgi membranes; and several populations with unknown subcellular origins. Most of the markers have complex distributions among the isolated membrane populations; this complexity is consistent with current concepts of the synthesis and recycling of membrane constituents and the regulation of cytosolic electrolyte activities.


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T. Yang, H. Zeng, J. Zhang, C. T. Okamoto, D. W. Warren, R. L. Wood, M. Bachmann, and A. K. Mircheff
MHC class II molecules, cathepsins, and La/SSB proteins in lacrimal acinar cell endomembranes
Am J Physiol Cell Physiol, November 1, 1999; 277(5): C994 - C1007.
[Abstract] [Full Text] [PDF]


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