AJP - Gastrointestinal and Liver Physiology, Vol 251, Issue 1 56-G63, Copyright © 1986 by American Physiological Society
Differences in pancreatic enzyme release from ventral and dorsal areas of the rat pancreas
R. Bruzzone, E. R. Trimble, A. Gjinovci and A. E. Renold
The exocrine secretory function of the ventral and dorsal areas of the rat
pancreas was investigated separately. In the isolated perfused rat
pancreas, caerulein (10(-12), 10(-11), and 10(-10) M) and carbachol (10(-7)
and 10(-6) M) caused a greaterfold increase in pancreatic secretion from
the dorsal than the ventral area. Caerulein (10(-9) M) and carbachol
(10(-5) M) were supramaximal concentrations with respect to secretion from
the dorsal area, but this was not the case in the ventral area. Similar
findings were also observed when ventral and dorsal secretions were studied
in vivo in anesthetized animals. By contrast, in dispersed acini the
sensitivity and responsiveness of tissue from the dorsal and ventral areas
were similar to both secretagogues. The possible influence of the endocrine
pancreas via the insuloacinar portal system was studied in the perfused
pancreas of diabetic rats treated with insulin in vivo. Carbachol (10(-6)
M)-stimulated secretion from the ventral area was similar (except for
amylase) to that of controls, while a significantly reduced (P less than
0.001) output was measured from the dorsal pancreas. These results
demonstrate that in the intact pancreas differences of exocrine secretion
exist between the ventral and dorsal areas and suggest that insulin may
contribute, at least in part, to the expression of such differences.
