AJP - Gastrointestinal and Liver Physiology, Vol 251, Issue 1 96-102, Copyright © 1986 by American Physiological Society
Actions of vasoactive intestinal peptide and secretin on chief cells prepared from guinea pig stomach
V. E. Sutliff, J. P. Raufman, R. T. Jensen and J. D. Gardner
Vasoactive intestinal peptide and secretin increased cellular cAMP and
pepsinogen secretion in dispersed chief cells from guinea pig gastric
mucosa. With each peptide there was a close correlation between the
dose-response curve for changes in cellular cAMP and that for changes in
pepsinogen secretion. Vasoactive intestinal peptide-(10-28) and
secretin-(5-27) had no agonist activity and antagonized the actions of
vasoactive intestinal peptide and secretin on cellular cAMP and pepsinogen
secretion. Studies of binding of 125I-vasoactive intestinal peptide and of
125I-secretin indicated that gastric chief cells possess four classes of
binding sites for vasoactive intestinal peptide and secretin and that
occupation of two of these classes of binding sites correlates with the
abilities of vasoactive intestinal peptide and secretin to increase
cellular cAMP and pepsinogen secretion. What function, if any, is mediated
by occupation by the other two classes of binding sites remains to be
determined.
