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Am J Physiol Gastrointest Liver Physiol (October 1, 2009). doi:10.1152/ajpgi.00342.2009
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Research Article

Galanin potentiates supramaximal caerulein-stimulated pancreatic amylase secretion via its action on somatostatin secretion

Savio George Barreto,1 Colin J Carati,1 Ann C Schloithe,1 James Toouli,2 and Gino Tony P Saccone1,*

1Flinders University 2Flinders Medical Center

Submitted 20 August 2009 ; revision received 16 September 2009 ; accepted in final form 17 September 2009

ABSTRACT

Galanin inhibits pancreatic amylase secretion from mouse lobules induced by physiological concentrations of caerulein via an insulin-dependent mechanism. We aimed to determine the effect and elucidate the mechanism of action of exogenous galanin on pancreatic amylase secretion induced by supramaximal concentrations of caerulein. Amylase secretion from isolated murine pancreatic lobules was measured. Lobules were co-incubated with galanin (10-12M-10-7M) and caerulein (10-7M). Lobules were pre-incubated with atropine (10-5M), tetrodotoxin (10-5M), diazoxide (10-7M), or the galanin antagonist galantide (10-12M-10-7M) for 30mins followed by incubation with caerulein alone, or combined with galanin (10-12M). Lobules were also co-incubated with combinations of galanin (10-12M), caerulein, octreotide (10-12M-10-7M) or cyclo-(7-aminoheptanoyl-Phe-D-Trp-Lys-Thr[BZL]), a somatostatin receptor antagonist (10-9M). Amylase secretion was expressed as percent of total lobular amylase. Caerulein stimulated amylase secretion to 124% of control. Diazoxide pre-treatment abolished the caerulein-stimulated amylase secretion while atropine or tetrodotoxin caused a partial inhibition. Galanin (10-12M-10-7M) potentiated caerulein-stimulated amylase secretion to 160% of control. Pre-incubation with a combination of atropine and diazoxide abolished galanin's potentiating effect indicating muscarinic receptor and insulin mediation. Pre-incubation with galantide abolished the galanin effect implying galanin receptor involvement. Co-incubation with caerulein, galanin and octreotide significantly reduced galanin's potentiating effect. However co-incubation with the somatostatin receptor antagonist, alone or in combination with galanin significantly increased caerulein-stimulated amylase secretion to a level comparable to the galanin potentiation. Taken together, these data suggest that at supramaximal caerulein concentrations, galanin acts via its receptors to further increase caerulein-stimulated amylase secretion by inhibiting the caerulein-induced release of somatostatin.

pancreatic exocrine secretion; caerulein; galanin; cholinergic


* Flinders University Gino.Saccone{at}flinders.edu.au






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