HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 285/6/G1111    most recent 00152.2003v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (22) Citing Articles via Google Scholar Google Scholar Articles by Strege, P. R. Articles by Farrugia, G. Search for Related Content PubMed PubMed Citation Articles by Strege, P. R. Articles by Farrugia, G.

NEUROREGULATION AND MOTILITY

Sodium current in human intestinal interstitial cells of Cajal

¹Enteric Neuroscience Program, ²Division of Gastroenterology and Hepatology, and ³Department of Surgery, Mayo Clinic and Mayo Foundation, Rochester, Minnesota 55905

Submitted 1 April 2003 ; accepted in final form 28 July 2003

Interstitial cells of Cajal (ICC) generate the electrical slow wave required for normal gastrointestinal motility. The ionic conductances expressed in human intestinal ICC are unknown. The aim of this study was to determine expression of a Na⁺ current in human intestinal ICC and to determine the effects of the Na⁺ current on the slow wave. Visually identified, freshly dissociated, single ICC were verified by the presence of c-kit mRNA by using single-cell RT-PCR. Standard whole cell currents were recorded from patch-clamped ICC held at -100 mV between pulse protocols. A Na⁺ current was identified in human intestinal ICC. The current activated at -55 mV and peaked at -30 mV. Extracellular N-methyl-D-glucamine abolished and QX-314 (500 µM) blocked the Na⁺ current, but nifedipine and Ni²⁺ did not. The Na⁺ current was activated by shear stress. Single-cell RT-PCR detected mRNA for the Na⁺ -subunit SCN5A in single human intestinal ICC. Lidocaine (200 µm) and QX-314 (500 µM) decreased slow wave frequency, and stretch increased slow wave frequency. A mechanosensitive Na⁺ channel current is present in human intestinal ICC and appears to play a role in the control of intestinal motor function.

pacemaker; small intestine; patch clamp; smooth muscle

This article has been cited by other articles:

				A. Corrias and M. L. Buist Quantitative cellular description of gastric slow wave activity Am J Physiol Gastrointest Liver Physiol, April 1, 2008; 294(4): G989 - G995. [Abstract] [Full Text] [PDF]

				C. E. Morris and P. F. Juranka Nav Channel Mechanosensitivity: Activation and Inactivation Accelerate Reversibly with Stretch Biophys. J., August 1, 2007; 93(3): 822 - 833. [Abstract] [Full Text] [PDF]

				H. Chen, D. Redelman, S. Ro, S. M. Ward, T. Ordog, and K. M. Sanders Selective labeling and isolation of functional classes of interstitial cells of Cajal of human and murine small intestine Am J Physiol Cell Physiol, January 1, 2007; 292(1): C497 - C507. [Abstract] [Full Text] [PDF]

				S. Nakayama, K. Shimono, H.-N. Liu, H. Jiko, N. Katayama, T. Tomita, and K. Goto Pacemaker phase shift in the absence of neural activity in guinea-pig stomach: a microelectrode array study J. Physiol., November 1, 2006; 576(3): 727 - 738. [Abstract] [Full Text] [PDF]

				M. Wouters, A. D. Laet, L. V. Donck, E. Delpire, P.-P. van Bogaert, J.-P. Timmermans, A. de Kerchove d'Exaerde, K. Smans, and J.-M. Vanderwinden Subtractive hybridization unravels a role for the ion cotransporter NKCC1 in the murine intestinal pacemaker Am J Physiol Gastrointest Liver Physiol, June 1, 2006; 290(6): G1219 - G1227. [Abstract] [Full Text] [PDF]

				P. R. Strege, C. E. Bernard, Y. Ou, S. J. Gibbons, and G. Farrugia Effect of mibefradil on sodium and calcium currents Am J Physiol Gastrointest Liver Physiol, August 1, 2005; 289(2): G249 - G253. [Abstract] [Full Text] [PDF]